USE OF BIOLOGICAL MARKERS FOR DETECTION OF THE ATYPICAL FLORA IN CHILDREN WITH RECURRENT BRONCHOPULMONARY DISEASES

Analyzes of the lymphocyte subpopulations (Table 2) revealed significantly the lowest CD3 lymphocyte count in the 1 subgroup of patients where the EN drug had a stimulating effect. Analysis of the parameters of circulating immune complexes in the subgroups of patients revealed their highest content also in 1 subgroup of patients (419±26,5) in comparison with the 2 and subgroups (305±28,9; 163±43,9). This indicates the highest tonsillogenic toxicity in patients of subgroup 1. Thus, the 1 subgroup of patients, where the stimulating effect of the drug EN on the NBT-test was revealed, was characterized by low level of T cell, highest rates of the CIC. Conclusions: the EN preparation showed a multidirectional immunological effect; the EN preparation acted in a stimulating manner on low indices, on high indices in a suppressive manner, on indices close to normal did not have any modulating effect; the EN preparation can be used to modulate the preimmune resistance indices in patients with chronic tonsillitis.

Analyzes of the lymphocyte subpopulations (Table 2) revealed significantly the lowest CD3 lymphocyte count in the 1 subgroup of patients where the EN drug had a stimulating effect. Analysis of the parameters of circulating immune complexes in the subgroups of patients revealed their highest content also in 1 subgroup of patients (419±26,5) in comparison with the 2 and subgroups (305±28,9; 163±43,9). This indicates the highest tonsillogenic toxicity in patients of subgroup 1.
Thus, the 1 subgroup of patients, where the stimulating effect of the drug EN on the NBT-test was revealed, was characterized by low level of T cell, highest rates of the CIC.
Conclusions: -the EN preparation showed a multidirectional immunological effect; -the EN preparation acted in a stimulating manner on low indices, on high indices -in a suppressive manner, on indices close to normal -did not have any modulating effect; -the EN preparation can be used to modulate the preimmune resistance indices in patients with chronic tonsillitis.

References:
1. Сахарова И.Я., Ариэль Б.М., Павлова М.В., Кноргинг Б.Е., Гусева И.Н. Специфический иммунный ответ и защитные факторы нейтрофильных гранулоцитов при легочном и внелегочном туберкулезе. Туберкулез и болезни легких. 2010. № 6. С. 20-24. Pathology of the respiratory system is the leading in the structure of childhood diseases. Recurrent bronchopulmonary pathology in children remains difficult for differentiating and requires use of combination of methods. The similarity and recurrence of symptoms of bronchitis caused by different health problems underlines importance of biological markers. The aim of the research was to assess roel of biological markers for evaluation of the role of the atypical flora in the course of recurrent bronchopulmonary diseases in children.

Alatas N., Baba F. Proliferating active cells, lymphocyte subsets, and dendritic cells in recurrent tonsillitis
Materials and methods. The study included 143 children who were admitted to the Zaporizhia Children's Multi-Profile Hospital due to recurrent bronchitis (3 and more episodes per year). Age of children ranged from 3 to 15 years. 77 patients were aged 3-7 years (53.8%), 66 patients -7-15 years -(46.2%). The ratio of male and female was approximately equal: 77 and 66 people (53.8 and 46.2%). Blood samples were taken according to the standard procedure. Immunoglobulines were detected with ELISA method. Bronchial asthma was verified following GINA guidelines.
Results: Analyzis of the historical and clinical data showed that in 43.5% of cases bronchitis occurred on a background of acute respiratory viral infection. 31.8% of patients had verified bronchial asthma, 24.2% -congenital malformations of the bronchi and lungs and 0.5% -cystic fibrosis. It was revealed that in patients with bronchial asthma in 43% of cases specific antibodies IgA, IgM and IgG to Cl. pneumoniae and M.pneumoniae were detected. That indicated presence of the atypical infection which could play a role in the course of the disease. Patients with uncontrolled asthma showed a high incidence of serological markers. More often IgM were detected as to Cl. pneumoniae, as to M.pneumoniae compared to IgA. 65% of children had IgM ans only 25% -IgA to Cl. pneumoniae and 75% of children had IgM ans only 35% -IgA M.pneumoniae.
Conclusions. Results of the study showed that specific antibodies to Cl. pneumoniae and M.pneumoniae were detected in 43% of children with bronchial asthma, which was verified in 31.8% of patients with recurrent symptoms of bronchitis. IgM atibodies were elevated more often than IgA.